Dakota Fanning
as Fern Arable

Julia Roberts
as Charlotte the Spider (voice)

Steve Buscemi
as Templeton the Rat (voice)

John Cleese
as Samuel the Sheep (voice)

Oprah Winfrey
as Gussy the Goose (voice)

Cedric the Entertainer
as Golly the Goose (voice)

Reba McEntire
as Betsy the Cow (voice)

Kathy Bates
as Bitsy the Cow (voice)

Robert Redford
as Ike the Horse (voice)

Thomas Haden Church
as Brooks the Crow (voice)

André 3000
as Elwyn the Crow (voice)

Dominic Scott Kay
as Wilbur (voice)

Sam Shepard
as Narrator (voice)

Abraham Benrubi
as Uncle the Pig (voice)

Kevin Anderson
as Mr. Arable

Essie Davis
as Mrs. Arable

Siobhan Fallon Hogan
as Mrs. Zuckerman

Louis Corbett
as Avery

Robyn Arthur
as Teacher

Julian O'Donnell
as Henry Fussy

Gary Basaraba
as Homer Zuckerman

Nate Mooney
as Lurvy

Nicholas Bell
as Minister

Beau Bridges
as Dr. Dorian

Teague Rook
as Well Dressed Man

Julia Zemiro
as Bystander

Denise Kirby
as New Teacher

Robert Plazek
as Ball Thrower

Joseph Lotesto
as Young Boy

Michael Roland
as Mayor

Don Bridges
as Announcer

Ian Watkin
as Fair Official

Joel McCrary
as Sheep Group (voice)

Brian Stepanek
as Sheep Group (voice)

Fred Tatasciore
as Sheep Group (voice)

Bradley White
as Sheep Group (voice)

Maia Kirkpatrick
as Joy the Baby Spider (voice)

Jennessa Rose
as Aranea the Baby Spider (voice)

Briana Hodge
as Nellie the Baby Spider (voice)

Dale Azzopardi
as Photographer (uncredited)

Ella Scott Lynch
as Girl at Fair (uncredited)