Humphrey Bogart
as Samuel Spade

Mary Astor
as Brigid O'Shaughnessy

Gladys George
as Iva Archer

Peter Lorre
as Joel Cairo

Barton MacLane
as Lt. of Detectives Dundy

Lee Patrick
as Effie Perine

Sydney Greenstreet
as Kasper Gutman

Ward Bond
as Det. Tom Polhaus

Jerome Cowan
as Miles Archer

Elisha Cook Jr.
as Wilmer Cook

James Burke
as Luke

Murray Alper
as Frank Richman

John Hamilton
as District Attorney Bryan

Charles Drake
as Reporter (uncredited)

Chester Gan
as Bit Part (uncredited)

Creighton Hale
as Stenographer (uncredited)

Robert Homans
as Policeman (uncredited)

William Hopper
as Reporter (uncredited)

Walter Huston
as Captain Jacoby (uncredited)

Hank Mann
as Reporter (uncredited)

Jack Mower
as Announcer (uncredited)

Emory Parnell
as Ship's Mate (uncredited)