Tobey Maguire
as Spider-Man / Peter Parker

Willem Dafoe
as Green Goblin / Norman Osborn

Kirsten Dunst
as Mary Jane Watson

James Franco
as Harry Osborn

Cliff Robertson
as Ben Parker

Rosemary Harris
as May Parker

J.K. Simmons
as J. Jonah Jameson

Joe Manganiello
as Flash Thompson

Gerry Becker
as Maximilian Fargas

Bill Nunn
as Joseph 'Robbie' Robertson

Jack Betts
as Henry Balkan

Stanley Anderson
as General Slocum

Ron Perkins
as Dr. Mendel Stromm

Michael Papajohn
as Carjacker

K.K. Dodds
as Simkins

Ted Raimi
as Hoffman

Bruce Campbell
as Ring Announcer

Elizabeth Banks
as Miss Brant

John Paxton
as Houseman

Tim DeZarn
as Philip Watson

Taylor Gilbert
as Madeline Watson

Randy Poffo
as Bone Saw McGraw

Larry Joshua
as Wrestling Promoter

Timothy Patrick Quill
as Wrestling Arena Guard

Lisa Danielle
as Bone-ette

Natalie T. Yeo
as Bone-ette

Erica D. Porter
as Bone-ette

Kristen Davidson
as Bone-ette

Jason Padgett
as Flash's Crony

Shan Omar Huey
as Teacher

Sally Levi
as Girl on Bus

Evan Arnold
as Doctor

Jill Sayre
as Nurse

Jim Ward
as Project Coordinator

David Holcomb
as Test Pilot

Octavia Spencer
as Check-In Girl

Brad Grunberg
as Heckler

Shane Habberstad
as Little Billy

Deborah Wakeham
as Billy's Mom

Rachael Bruce
as Times Square Child

Mackenzie Bryce
as Times Square Child

Julia Barrie
as Times Square Child

Macy Gray
as Macy Gray

Myk Watford
as Cop at Fire

Bill Calvert
as Fireman

Sylva Kelegian
as Mother at Fire

Kristen Marie Holly
as Young Lady at Fire

Ajay Mehta
as Cabbie

Peter Appel
as Cabbie

Scott Spiegel
as Marine Cop

Matt Smith
as Cop at Carjacking

Sara Ramirez
as Cop at Carjacking

Lucy Lawless
as Punk Rock Girl

Jayce Bartok
as Subway Guitarist

Maribel González
as Lady Dogwalker

Amy Bouril
as Office Lady

Joseph D'Onofrio
as Opinionated Cop

Jim Norton
as Surly Truck Driver

Corey Mendell Parker
as Chaperone in Tram

Ashley Edner
as Girl in Tram

William Joseph Firth
as Boy in Tram

Alex Black
as Boy in Tram

Laura Gray
as Tram Group Mother

Joe Virzi
as New Yorker on Bridge

Michael Edward Thomas
as New Yorker on Bridge

Jeanie Fox
as New Yorker on Bridge

Robert Kerman
as Tugboat Captain

Stan Lee
as Man in Fair (uncredited)

Una Damon
as Lab Tour Guide (uncredited)

Rick Avery
as Cop (uncredited)

Peter Aylward
as Colonel (uncredited)

Jillian Clare
as Crying Girl in Tram (uncredited)

Chris Coppola
as Kyle (uncredited)

Jesse Heiman
as Spectator at School Fight (uncredited)

Leroy Patterson
as World Unity Festival Attendant (uncredited)

Benny Urquidez
as Mugger (uncredited)

Scott L. Schwartz
as Screaming Wrestler (uncredited)

Jophery C. Brown
as Chef (uncredited)

Brian J. Williams
as Young Thug #2 (uncredited)

Mark De Alessandro
as Cop (uncredited)

Tia Dionne Hodge
as Uptown Woman (uncredited)

Loren Janes
as Board of Directors Member (uncredited)

Andray Johnson
as Balkan's Aide (uncredited)

Martin Pfefferkorn
as Thug on 8x10 (uncredited)

Tammi Sutton
as Street Vendor (uncredited)

Lindsay Thompson
as Mary Jane's Friend (uncredited)

Sean Valla
as Boat Light Man (uncredited)

Pete Macnamara
as Pentagon Officer (uncredited)

Al Goto
as Cop (uncredited)

Joni Avery
as Cop (uncredited)

Sam Raimi
as Popcorn Thrower (uncredited)

Craig "Radioman" Castaldo
as Man with Bike (uncredited)